Human Beta Cell Lines
Research
The EndoC βH cell lines represent powerful tools to study functional human beta cells.
We have produced 3 generations of the EndoC βH cell line used by over 150 research groups across the world.
Published Work
2019
Stearoyl CoA desaturase is a gatekeeper that protects human ß cells against lipotoxicity and maintains their identity. Oshima M, Pechberty S, Bellini L, Göpel S, Campana M2, Rouch C, Dairou J, Cosentino C, Fantuzzi F, Toivonen S, Marchetti P, Magnan C, Cnop M, Le Stunff H, Scharfmann R. Diabetologia 2019 Dec 3. PMID : 31796987
The microRNAs miR-211-5p and miR-204-5p modulate ER stress in human beta cells. Grieco FA, Schiavo AA, Brozzi F, Juan-Mateu J, Bugliani M, Marchetti P, Eizirik D. J Mol Endocrinol. 2019 Jul 1. pii: JME-19-0066.R1. doi: 10.1530/JME-19-0066. [Epub ahead of print] PMID: 31277072
Development of a New Mouse Model for Coxsackievirus-Induced Myocarditis by Attenuating Coxsackievirus B3 Virulence in the Pancreas. Pinkert S, Pryshliak M, Pappritz K, Knoch K, Hazini A, Dieringer B, Schaar K, Dong F, Hinze L, Lin J, Lassner D, Klopfleisch R, Solimena M, Tschöpe C, Kaya Z, Beling A, Kurreck J, van Linthout S, Klingel K, Fechner H. Cardiovasc Res. 2019 Oct 9. pii: cvz259. doi: 10.1093/cvr/cvz259. [Epub ahead of print] PMID: 31598635
A CRISPR/Cas9 genome editing pipeline in the EndoC-βH1 cell line to study genes implicated in beta cell function. Grotz AK, Abaitua F, Navarro-Guerrero E, Hastoy B, Ebner D, Gloyn AL. [version 1; peer review: 1 approved, 1 approved with reservations]. Wellcome Open Res 2019, 4:150
MiR-375-mediated suppression of engineered coxsackievirus B3 in pancreatic cells. Pryshliak M, Hazini A, Knoch K, Dieringer B, Tolksdorf B, Solimena M, Kurreck J, Pinkert S, Fechner H. FEBS Lett. 2019 Oct 23. PMID: 31643074
Loss-of-function mutations in MRAP2 are pathogenic in hyperphagic obesity with hyperglycemia and hypertension. Baron M, Maillet J, Huyvaert M, Dechaume A, Boutry R, Loiselle H, Durand E, Toussaint B, Vaillant E, Philippe J, Thomas J, Ghulam A, Franc S, Charpentier G, Borys JM, Lévy-Marchal C, Tauber M, Scharfmann R, Weill J, Aubert C, Kerr-Conte J, Pattou F, Roussel R, Balkau B, Marre M, Boissel M, Derhourhi M, Gaget S, Canouil M, Froguel P, Bonnefond A. Nat Med. 2019 25(11):1733-1738. PMID: 31700171
iPreP is a three-dimensional nanofibrillar cellulose hydrogel platform for long-term ex vivo preservation of human islets. Chen YJ, Yamazoe T, Leavens KF, Cardenas-Diaz FL, Georgescu A, Huh D, Gadue P, Stanger BZ. JCI Insight. 2019 Nov 1;4(21). pii: 124644. PMID: 31672937
Down-regulation of the islet-specific zinc transporter-8 (ZnT8) protects human insulinoma cells against inflammatory stress. Merriman C, Fu D. J Biol Chem. 2019 294(45):16992-17006. PMID : 31591269
Delineating the role of FANCA in glucose-stimulated insulin secretion in β cells through its protein interactome. Lagundžin D, Hu WF, Law HCH, Krieger KL, Qiao F, Clement EJ, Drincic AT, Nedić O, Naldrett MJ, Alvarez S, Woods NT. PLoS One. 2019 14(8):e0220568. PMID : 31461451
Modeling Monogenic Diabetes using Human ESCs Reveals Developmental and Metabolic Deficiencies Caused by Mutations in HNF1A. Cardenas-Diaz FL, Osorio-Quintero C, Diaz-Miranda MA, Kishore S, Leavens K, Jobaliya C, Stanescu D, Ortiz-Gonzalez X, Yoon C, Chen CS, Haliyur R, Brissova M, Powers AC, French DL, Gadue P.Cell Stem Cell. 2019 Aug 1;25(2):273-289. PMID: 31374199
Early Detection of Peripheral Blood Cell Signature in Children Developing β-Cell Autoimmunity at a Young Age. Kallionpää H, Somani J, Tuomela S, Ullah U, de Albuquerque R, Lönnberg T, Komsi E, Siljander H, Honkanen J, Härkönen T, Peet A, Tillmann V, Chandra V, Anagandula MK, Frisk G, Otonkoski T, Rasool O, Lund R, Lähdesmäki H, Knip M, Lahesmaa R. Diabetes. 2019 68(10):2024-2034. PMID: 31311800
Structure-toxicity relationships of saturated and unsaturated free fatty acids for elucidating the lipotoxic effects in human EndoC-βH1 beta-cells. Plötz T, von Hanstein AS, Krümmel B, Laporte A, Mehmeti I, Lenzen S.Biochim Biophys Acta Mol Basis Dis. 2019 Aug 6;1865(11):165525. PMID: 31398470
Glucolipotoxicity Alters Insulin Secretion via Epigenetic Changes in Human Islets. Hall E, Jönsson J, Ofori JK, Volkov P, Perfilyev A, Dekker-Nitert M, Eliasson L, Ling C, Bacos K. Diabetes. 2019 68(10):1965-1974. PMID: 31420409
m6A mRNA Methylation Regulates Human β-Cell Biology in Physiological States and in Type 2 Diabetes. De Jesus DF, Zhang Z, Kahraman S, Brown NK, Chen M, Hu J, Gupta MK, He C, Kulkarni RN. Nat Metab. 2019;1(8):765-774. PMID: 31867565
Human pancreatic islet three-dimensional chromatin architecture provides insights into the genetics of type 2 diabetes. Miguel-Escalada I, Bonàs-Guarch S, Cebola I, Ponsa-Cobas J, Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, Morgan CC, García-Hurtado J, Beucher A, Morán I, Pasquali L, Ramos-Rodríguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J.Nat Genet. 2019 Jul;51(7):1137-1148. PMID: 31253982
Cellular stressors may alter islet hormone cell proportions by moderation of alternative splicing patterns. Jeffery N, Richardson S, Chambers D, Morgan NG, Harries LW.Hum Mol Genet. 2019 Aug 15;28(16):2763-2774. PMID: 31098640
A novel RT-QPCR-based assay for the relative quantification of residue specific m6A RNA methylation. Castellanos-Rubio A, Santin I, Olazagoitia-Garmendia A, Romero-Garmendia I, Jauregi-Miguel A, Legarda M, Bilbao JR. Sci Rep. 2019 Mar 12;9(1):4220. PMID: 30862814
Coxsackievirus B Tailors the Unfolded Protein Response to Favour Viral Amplification in Pancreatic β Cells. Colli ML, Paula FM, Marselli L, Marchetti P, Roivainen M, Eizirik DL, Op de Beeck A.J Innate Immun. 2019 Feb 20:1-15. doi: 10.1159/000496034. PMID: 30799417
Cytokine-induced translocation of GRP78 to the plasma membrane triggers a pro-apoptotic feedback loop in pancreatic beta cells. Vig S, Buitinga M, Rondas D, Crèvecoeur I, van Zandvoort M, Waelkens E, Eizirik DL, Gysemans C, Baatsen P, Mathieu C, Overbergh L.Cell Death Dis. 2019 Apr 5;10(4):309. PMID: 30952835
Pancreatic β-cells detoxify H2O2 through the peroxiredoxin/thioredoxin antioxidant system. Stancill JS, Broniowska KA, Oleson BJ, Naatz A, Corbett JA. J Biol Chem. 2019 Mar 29;294(13):4843-4853. PMID: 30659092
ICA512 RESP18 homology domain is a protein condensing factor and insulin fibrillation inhibitor. Toledo PL, Torkko JM, Müller A, Wegbrod C, Sönmez A, Solimena M, Ermácora MR. J Biol Chem. 2019 May 24;294(21):8564-8576.doi: 10.1074/jbc.RA119.007607. PMID: 30979722
Multiomic Profiling Identifies cis-Regulatory Networks Underlying Human Pancreatic β Cell Identity and Function. Lawlor N, Márquez EJ, Orchard P, Narisu N, Shamim MS, Thibodeau A, Varshney A, Kursawe R, Erdos MR, Kanke M, Gu H, Pak E, Dutra A, Russell S, Li X, Piecuch E, Luo O, Chines PS, Fuchbserger C; NIH Intramural Sequencing Center, Sethupathy P, Aiden AP, Ruan Y, Aiden EL, Collins FS, Ucar D, Parker SCJ, Stitzel ML. Cell Rep. 2019 Jan 15;26(3):788-801.e6. doi: 10.1016/j.celrep.2018.12.083. PMID: 30650367
MCPIP1 regulates the sensitivity of pancreatic beta-cells to cytokine toxicity. Tyka K, Jörns A, Turatsinze JV, Eizirik DL, Lenzen S, Gurgul-Convey E. Cell Death Dis. 2019 Jan 10;10(1):29. doi: 10.1038/s41419-018-1268-4. PMID: 30631045
Inhibition of Eph/ephrin interaction with the small molecule UniPR500 improves glucose tolerance in healthy and insulin-resistant mice. Giorgio C, Incerti M, Pala D, Russo S, Chiodelli P, Rusnati M, Cantoni AM, Di Lecce R, Barocelli E, Bertoni S, Ravassard P, Manenti F, Piemonti L, Ferlenghi F, Lodola A, Tognolini M. Pharmacol Res. 2019 Jan 5. pii: S1043-6618(18)30822-3. PMID: 30625359
The transcription factor STAT6 plays a critical role in promoting beta cell viability and is depleted in islets of individuals with type 1 diabetes. Leslie KA, Russell MA, Taniguchi K, Richardson SJ, Morgan NG Diabetologia. 2019 Jan;62(1):87-98. doi: 10.1007/s00125-018-4750-8. PMID: 30338340
The impact of proinflammatory cytokines on the β-cell regulatory landscape provides insights into the genetics of type 1 diabetes. Ramos-Rodríguez M, Raurell-Vila H, Colli ML, Alvelos MI, Subirana-Granés M, Juan-Mateu J, Norris R, Turatsinze JV, Nakayasu ES, Webb-Robertson BM, Inshaw JRJ, Marchetti P, Piemonti L, Esteller M, Todd JA, Metz TO, Eizirik DL, Pasquali L. Nat Genet. 2019 51(11):1588-1595. PMID: 31676868
DEXI, a candidate gene for type 1 diabetes, modulates rat and human pancreatic beta cell inflammation via regulation of the type I IFN/STAT signalling pathway. Dos Santos RS, Marroqui L, Velayos T, Olazagoitia-Garmendia A, Jauregi-Miguel A, Castellanos-Rubio A, Eizirik DL, Castaño L, Santin I. Diabetologia. 2019 Mar;62(3):459-472. doi: 10.1007/s00125-018-4782-0. PMID: 30478640
Boosting to Amplify Signal with Isobaric Labeling (BASIL) Strategy for Comprehensive Quantitative Phosphoproteomic Characterization of Small Populations of Cells. Yi L, Tsai CF, Dirice E, Swensen AC, Chen J, Shi T, Gritsenko MA, Chu RK, Piehowski PD, Smith RD, Rodland KD, Atkinson MA, Mathews CE, Kulkarni RN, Liu T, Qian WJ.Anal Chem. 2019 91(9):5794-5801. doi: 10.1021/acs.analchem.9b00024. PMID: 30843680
Loss of ZnT8 function protects against diabetes by enhanced insulin secretion. Dwivedi OP, Lehtovirta M, Hastoy B, Chandra V, Krentz NAJ, Kleiner S, Jain D, Richard AM, Abaitua F, Beer NL, Grotz A, Prasad RB, Hansson O, Ahlqvist E, Krus U, Artner I, Suoranta A, Gomez D, Baras A, Champon B, Payne AJ, Moralli D, Thomsen SK, Kramer P, Spiliotis I, Ramracheya R, Chabosseau P, Theodoulou A, Cheung R, van de Bunt M, Flannick J, Trombetta M, Bonora E, Wolheim CB, Sarelin L, Bonadonna RC, Rorsman P, Davies B, Brosnan J, McCarthy MI, Otonkoski T, Lagerstedt JO, Rutter GA, Gromada J, Gloyn AL, Tuomi T, Groop L. Nat Genet. 2019 Nov;51(11):1596-1606. PMID: 31676859
BiFET: sequencing Bias-free transcription factor Footprint Enrichment Test. Youn A, Marquez EJ, Lawlor N, Stitzel ML, Ucar D. Nucleic Acids Res. 2019 47(2):e11. doi: 10.1093/nar/gky1117. PMID: 30428075
New α- and SIN γ-retrovectors for safe transduction and specific transgene expression in pancreatic β cell lines. Albagli O, Maugein A, Huijbregts L, Bredel D, Carlier G, Martin P, Scharfmann R. BMC Biotechnol. 2019 19(1):35. PMID: 31208395
HNF4A Haploinsufficiency in MODY1 Abrogates Liver and Pancreas Differentiation from Patient-Derived Induced Pluripotent Stem Cells. Ng NHJ, Jasmen JB, Lim CS, Lau HH, Krishnan VG, Kadiwala J, Kulkarni RN, Ræder H, Vallier L, Hoon S, Teo AKK. iScience. 2019 ;16:192-205. PMID: 31195238
2018
Syntaxin 4 Expression in Pancreatic β-Cells Promotes Islet Function and Protects Functional β-Cell Mass. Oh E, Ahn M, Afelik S, Becker TC, Roep BO, Thurmond DC. Diabetes. 2018 Dec;67(12):2626-2639. PMID: 30305365
Vegf-A mRNA transfection as a novel approach to improve mouse and human islet graft revascularisation. Staels W, Verdonck Y, Heremans Y, Leuckx G, De Groef S, Heirman C, de Koning E, Gysemans C, Thielemans K, Baeyens L, Heimberg H, De Leu N. Diabetologia. 2018 Aug;61(8):1804-1810. doi: 10.1007/s00125-018-4646-7. Epub 2018 May 22. PMID: 29789879
Exploring the insulin secretory properties of the PGD2-GPR44/DP2 axis in vitro and in a randomized phase-1 trial of type 2 diabetes patients. Skrtic S, Tyrberg B, Broberg M, Ericsson H, Schnecke V, Kjaer M, Hompesch M, Andersson EM, Ryberg E, Aivazidis A, Wennberg Huldt C, Löfgren L, Morrow L, Parkinson J, Rydén-Bergsten T, Watkins E, Sörhede Winzell M.PLoS One. 2018 Dec 17;13(12):e0208998 PMID: 30557325
Bioluminescent reporter assay for monitoring ER stress in human beta cells. Kracht MJL, de Koning EJP, Hoeben RC, Roep BO, Zaldumbide A. Sci Rep. 2018 Dec 10;8(1):17738. doi: 10.1038/s41598-018-36142-4.PMID: 30532033
Improved Baculovirus Vectors for Transduction and Gene Expression in Human Pancreatic Islet Cells. Graves LP, Aksular M, Alakeely RA, Ruiz Buck D, Chambers AC, Murguia-Meca F, Plata-Muñoz JJ, Hughes S, Johnson PRV, Possee RD, King LA. Viruses. 2018 Oct 20;10(10). pii: E574. PMID: 30347797
Beta cell extracellular vesicle miR-21-5p cargo is increased in response to inflammatory cytokines and serves as a biomarker of type 1 diabetes. Lakhter AJ, Pratt RE, Moore RE, Doucette KK, Maier BF, DiMeglio LA, Sims EK. Diabetologia. 2018 May;61(5):1124-1134. PMID: 30372121
The protein synthesis inhibitor brusatol normalizes high-fat diet-induced glucose intolerance in male C57BL/6 mice: role of translation factor eIF5A hypusination. Turpaev K, Krizhanovskii C, Wang X, Sargsyan E, Bergsten P, Welsh N. FASEB J. 2018 Nov 21:fj201801698R. doi: 10.1096/fj.201801698R. PMID: 30462531
A neural network based model effectively predicts enhancers from clinical ATAC-seq samples. Thibodeau A, Uyar A, Khetan S, Stitzel ML, Ucar D. Sci Rep. 2018 Oct 30;8(1):16048. doi: 10.1038/s41598-018-34420-9. PMID: 30375457
PDL1 is expressed in the islets of people with type 1 diabetes and is up-regulated by interferons-α and-γ via IRF1 induction. Colli ML, Hill JLE, Marroquí L, Chaffey J, Dos Santos RS, Leete P, Coomans de Brachène A, Paula FMM, Op de Beeck A, Castela A, Marselli L, Krogvold L, Dahl-Jorgensen K, Marchetti P, Morgan NG, Richardson SJ, Eizirik DL. EBioMedicine. 2018 Oct;36:367-375.
Electrophysiological properties of human beta-cell lines EndoC-βH1 and -βH2 conform with human beta-cells. Hastoy B, Godazgar M, Clark A, Nylander V, Spiliotis I, van de Bunt M, Chibalina MV, Barrett A, Burrows C, Tarasov AI, Scharfmann R, Gloyn AL, Rorsman P. Sci Rep. 2018 Nov 19;8(1):16994
A hPSC-based platform to discover gene-environment interactions that impact human β-cell and dopamine neuron survival. Zhou T, Kim TW, Chong CN, Tan L, Amin S, Sadat Badieyan Z, Mukherjee S, Ghazizadeh Z, Zeng H, Guo M, Crespo M, Zhang T, Kenyon R, Robinson CL, Apostolou E, Wang H, Xiang JZ, Evans T, Studer L, Chen S. Nat Commun. 2018 Nov 16;9(1):4815. doi: 10.1038/s41467-018-07201-1.
LY3298176, a novel dual GIP and GLP-1 receptor agonist for the treatment of type 2 diabetes mellitus: From discovery to clinical proof of concept. Coskun T, Sloop KW, Loghin C, Alsina-Fernandez J, Urva S, Bokvist KB, Cui X, Briere DA, Cabrera O, Roell WC, Kuchibhotla U, Moyers JS, Benson CT, Gimeno RE, D'Alessio DA, Haupt A. Mol Metab. 2018 Dec;18:3-14. doi: 10.1016/j.molmet.2018.09.009. PMID: 30473097
The novel NADPH oxidase 4 selective inhibitor GLX7013114 counteracts human islet cell death in vitro. Wang X, Elksnis A, Wikström P, Walum E, Welsh N, Carlsson PO. PLoS One. 2018 Sep 28;13(9):e0204271.
Differentiation of Sendai virus-reprogrammed iPSC into β cells, compared with human pancreatic islets and immortalized β cell line. Pellegrini S, Manenti F, Chimienti R, Nano R, Ottoboni L, Ruffini F, Martino G, Ravassard P, Piemonti L, Sordi V. Cell Transplant. Cell Transplant. 2018 Oct;27(10):1548-1560. doi: 10.1177/0963689718798564. PMID: 30251567
Pancreatic β-cell tRNA hypomethylation and fragmentation link TRMT10A deficiency with diabetes. Cosentino C, Toivonen S, Diaz Villamil E, Atta M, Ravanat JL, Demine S, Schiavo AA, Pachera N, Deglasse JP, Jonas JC, Balboa D, Otonkoski T, Pearson ER, Marchetti P, Eizirik DL, Cnop M, Igoillo-Esteve M. Nucleic Acids Res. 2018 Nov 2;46(19):10302-10318. doi: 10.1093/nar/gky839. PMID: 30247717
Highly specific monoclonal antibodies for allosteric inhibition and immunodetection of the human pancreatic zinc transporter ZnT8. Merriman C, Li H, Li H, Fu D. J Biol Chem. 2018 Oct 19;293(42):16206-16216. doi: 10.1074/jbc.RA118.005136. PMID: 30181214
Imaging of Human Insulin Secreting Cells with Gd-DOTA-P88, a Paramagnetic Contrast Agent Targeting the Beta Cell Biomarker FXYD2γa. Demine S, Balhuizen A, Debaille V, Joosten L, Fereau M, Chilla SNM, Millard I, Scharfmann R, Egrise D, Goldman S, Marchetti P, Gotthardt M, Laurent S, Burtea C, Eizirik DL.Molecules. 2018 Aug 21;23(9). pii: E2100. doi: 10.3390/molecules23092100. PMID: 30134599
Conventional and Neo-Antigenic Peptides Presented by β Cells Are Targeted by Circulating Naïve CD8+ T Cells in Type 1 Diabetic and Healthy Donors. Gonzalez-Duque S, Azoury ME, Colli ML, Afonso G, Turatsinze J-V, Nigi L, Lalanne AI, Sebastiani G, Carré A, Pinto S, Culina S, Corcos N, Bugliani M, Marchetti P, Armanet M, Diedisheim M, Kyewski B, Steinmetz LM, Buus S, You S, Dubois-Laforgue D, Larger E, Beressi J-P, Bruno G, Dotta F, Scharfmann R, Eizirik DL, Verdier Y, Vinh J, Mallone R. Cell Metab. 2018 Dec 4;28(6):946-960.e6. doi: 10.1016/j.cmet.2018.07.007. PMID: 30078552
Unexpected subcellular distribution of a specific isoform of the Coxsackie and adenovirus receptor, CAR-SIV, in human pancreatic beta cells. Ifie E, Russell MA, Dhayal S, Leete P, Sebastiani G, Nigi L, Dotta F, Marjomäki V, Eizirik DL, Morgan NG, Richardson SJ.Diabetologia. 2018 Nov;61(11):2344-2355. doi: 10.1007/s00125-018-4704-1. PMID: 30074059
Type 2 diabetes risk alleles in PAM impact insulin release from human pancreatic β-cells. Thomsen SK, Raimondo A, Hastoy B, Sengupta S, Dai XQ, Bautista A, Censin J, Payne AJ, Umapathysivam MM, Spigelman AF, Barrett A, Groves CJ, Beer NL, Manning Fox JE, McCarthy MI, Clark A, Mahajan A, Rorsman P, MacDonald PE, Gloyn AL. Nat Genet. 2018 Nov;50(11):1505-1513. doi: 10.1038/s41588-018-0241-6. Epub 2018 Oct 8. PMID: 30297969
Integration of human pancreatic islet genomic data refines regulatory mechanisms at Type 2 Diabetes susceptibility loci. Thurner M, van de Bunt M, Torres JM, Mahajan , Nylander V2, Bennett AJ, Gaulton KJ, Barrett A, Burrows C, Bell CG, Lowe R, Beck S, Rakyan VK, Gloyn AL, McCarthy MI. Elife. 2018 Feb 7;7. pii: e31977. doi: 10.7554/eLife.31977. PMID: 29412141
The Effects of Kisspeptin on β-cell Function, Serum Metabolites and Appetite in Humans. Izzi-Engbeaya C, Comninos AN, Clarke SA, Jomard A, Yang L, Jones S, Abbara A, Narayanaswamy S, Eng PC, Papadopoulou D, Prague JK, Bech P, Godsland IF, Bassett P, Sands C, Camuzeaux S, Gomez Romero M, Pearce JTM, Lewis MR, Holmes E, Nicholson JK, Tan T, Ratnasabapathy R, Hu M, Carrat G, Piemonti L, Bugliani M, Marchetti P, Johnson PR, Hughes SJ, Shapiro AMJ, Rutter GA, Dhillo WS. Diabetes Obes Metab. 2018 Dec;20(12):2800-2810. doi: 10.1111/dom.13460. PMID: 29974637
Human EndoC-βH1 β-cells form pseudoislets with improved glucose sensitivity and enhanced GLP-1 signaling in the presence of islet-derived endothelial cells. Spelios MG, Afinowicz LA, Tipon RC, Akirav EM. Am J Physiol Endocrinol Metab. 2018 314:E512-E521
Acute and Repeated Treatment with 5-PAHSA or 9-PAHSA Isomers Does Not Improve Glucose Control in Mice. Pflimlin E, Bielohuby M, Korn M, Breitschopf K, Löhn M, Wohlfart P, Konkar A, Podeschwa M, Bärenz F, Pfenninger A, Schwahn U, Opatz T, Reimann M, Petry S, Tennagels N. Cell Metab. 2018 Aug 7;28(2):217-227.e13. doi: 10.1016/j.cmet.2018.05.028. PMID: 29937376
MANF protects human pancreatic beta cells against stress-induced cell death. Hakonen E, Chandra V, Fogarty CL, Yu NY, Ustinov J, Katayama S, Galli E, Danilova T, Lindholm P, Vartiainen A, Einarsdottir E, Krjutškov K, Kere J, Saarma M, Lindahl M, Otonkoski T. Diabetologia. 2018 Oct;61(10):2202-2214. doi: 10.1007/s00125-018-4687-y. PMID: 30032427
MAFA missense mutation causes familial insulinomatosis and diabetes mellitus. Iacovazzo D, Flanagan SE, Walker E, Quezado R, de Sousa Barros FA, Caswell R, Johnson MB, Wakeling M, Brändle M, Guo M, Dang MN, Gabrovska P, Niederle B, Christ E, Jenni S, Sipos B, Nieser M, Frilling A, Dhatariya K, Chanson P, de Herder WW, Konukiewitz B, Klöppel G, Stein R, Korbonits M, and Ellard S. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):1027-1032.
Insulin promoter in human pancreatic β cells contacts diabetes susceptibility loci and regulates genes affecting insulin metabolism. Jian X, Felsenfeld G. Proc Natl Acad Sci U S A. 2018 May 15;115(20):E4633-E4641.
Synaptotagmin 4 Regulates Pancreatic β Cell Maturation by Modulating the Ca2+ Sensitivity of Insulin Secretion Vesicles. Huang C, Walker EM, Dadi PK, Hu R, Xu Y, Zhang W, Sanavia T, Mun J, Liu J, Nair GG, Tan HYA, Wang S, Magnuson MA, Stoeckert CJ Jr, Hebrok M, Gannon M, Han W7 Stein R, Jacobson DA, Gu G. Dev Cell. 2018 May 7;45(3):347-361.
20-HETE promotes glucose-stimulated insulin secretion in an autocrine manner through FFAR1. Tunaru S, Bonnavion R, Brandenburger I, Preussner J, Thomas D, Scholich K, Offermanns S. Nat Commun. 2018 Jan 12;9(1):177
Targeting GLP-1 receptor trafficking to improve agonist efficacy. Jones B, Buenaventura T, Kanda N, Chabosseau P, Owen BM, Scott R, Goldin R, Angkathunyakul N, Corrêa IR Jr, Bosco D, Johnson PR, Piemonti L, Marchetti P, Shapiro AMJ, Cochran BJ, Hanyaloglu AC, Inoue A, Tan T, Rutter GA, Tomas A, Bloom SR. Nat Commun. 2018 Apr 23;9(1):1602.
BRICHOS domain of Bri2 inhibits islet amyloid polypeptide (IAPP) fibril formation and toxicity in human beta cells. Oskarsson ME, Hermansson E, Wang Y, Welsh N, Presto J, Johansson J, Westermark GT. Proc Natl Acad Sci U S A. 2018 Mar 20;115(12):E2752-E2761
Secretagogin is increased in plasma from type 2 diabetes patients and potentially reflects stress and islet dysfunction. Hansson SF, Zhou AX, Vachet P, Eriksson JW, Pereira MJ, Skrtic S, Jongsma Wallin H, Ericsson-Dahlstrand A, Karlsson D, Ahnmark A, Sörhede Winzell M, Magnone MC, Davidsson P. PLoS One. 2018 Apr 27;13(4):e0196601
Modeling human pancreatic beta cell dedifferentiation. Diedisheim M, Oshima M, Albagli O, Huldt CW, Ahlstedt I, Clausen M, Menon S, Aivazidis A, Andreasson AC, Haynes WG, Marchetti P, Marselli L, Armanet M, Chimienti F, Scharfmann R. Mol Metab. 2018 Apr;10:74-86
Systems biology of the IMIDIA biobank from organ donors and pancreatectomised patients defines a novel transcriptomic signature of islets from individuals with type 2 diabetes. Solimena M, Schulte AM, Marselli L, Ehehalt F, Richter D, Kleeberg M, Mziaut H, Knoch KP, Parnis J, Bugliani M, Siddiq A, Jörns A, Burdet F, Liechti R, Suleiman M, Margerie D, Syed F, Distler M, Grützmann R, Petretto E, Moreno-Moral A, Wegbrod C, Sönmez A, Pfriem K, Friedrich A, Meinel J, Wollheim CB, Baretton GB, Scharfmann R, Nogoceke E, Bonifacio E, Sturm D, Meyer-Puttlitz B, Boggi U, Saeger HD, Filipponi F, Lesche M, Meda P, Dahl A, Wigger L, Xenarios I, Falchi M, Thorens B, Weitz J, Bokvist K, Lenzen S, Rutter GA, Froguel P, von Bülow M, Ibberson M, Marchetti P. Diabetologia. 2018 Mar;61(3):641-657
DPP-4 is expressed in human pancreatic beta cells and its direct inhibition improves beta cell function and survival in type 2 diabetes. Bugliani M, Syed F, Paula FMM, Omar BA, Suleiman M, Mossuto S, Grano F, Cardarelli F, Boggi U, Vistoli F, Filipponi F, De Simone P, Marselli L, De Tata V, Ahren B, Eizirik DL, Marchetti P. Mol Cell Endocrinol. 2018 473:186-193
MondoA Is an Essential Glucose-Responsive Transcription Factor in Human Pancreatic β-Cells. Richards P, Rachdi L, Oshima M, Marchetti P, Bugliani M, Armanet M, Postic C, Guilmeau S, Scharfmann R. Diabetes. 2018 Mar;67(3):461-472.
Role of Protein Phosphatase 1 and Inhibitor of Protein Phosphatase 1 in Nitric Oxide–Dependent Inhibition of the DNA Damage Response in Pancreatic β-Cells. Oleson BJ, Naatz A, Proudfoot SC, Yeo CT, Corbett JA. Diabetes. 2018 May;67(5):898-910
Islet-reactive CD8+ T cell frequencies in the pancreas, but not in blood, distinguish type 1 diabetic patients from healthy donors. Culina S, Lalanne AI, Afonso G, Cerosaletti K, Pinto S, Sebastiani G, Kuranda K, Nigi L, Eugster A, Østerbye T, Maugein A, McLaren JE, Ladell K, Larger E, Beressi JP, Lissina A, Appay V, Davidson HW, Buus S, Price DA, Kuhn M, Bonifacio E, Battaglia M, Caillat-Zucman S, Dotta F, Scharfmann R, Kyewski B, Mallone R. Sci Immunol. 2018 Feb 2;3(20). pii: eaao4013. doi: 10.1126/sciimmunol.aao4013. PMID: 29429978
Virus-like infection induces human β cell dedifferentiation. Oshima M, Knoch KP, Diedisheim M, Petzold A, Cattan P, Bugliani M, Marchetti P, Choudhary P, Huang GC, Bornstein SR, Solimena M, Albagli-Curiel O, Scharfmann R. JCI Insight. 2018 Feb 8;3(3). pii: 97732. doi: 10.1172/jci.insight.97732. PMID: 29415896
The EndoC-βH1 cell line is a valid model of human beta cells and applicable for screenings to identify novel drug target candidates. Tsonkova VG, Sand FW, Wolf XA, Grunnet LG, Kirstine Ringgaard A, Ingvorsen C, Winkel L, Kalisz M, Dalgaard K, Bruun C, Fels JJ, Helgstrand C, Hastrup S, Öberg FK, Vernet E, Sandrini MPB, Shaw AC, Jessen C, Grønborg M, Hald J, Willenbrock H, Madsen D, Wernersson R, Hansson L, Jensen JN, Plesner A, Alanentalo T, Petersen MBK, Grapin-Botton A, Honoré C, Ahnfelt-Rønne J, Hecksher-Sørensen J, Ravassard P, Madsen OD, Rescan C, Frogne T. Mol Metab. 2018 Feb;8:144-157
Exercise training protects human and rodent β cells against endoplasmic reticulum stress and apoptosis. Paula FMM, Leite NC, Borck PC, Freitas-Dias R, Cnop M, Chacon-Mikahil MPT, Cavaglieri CR, Marchetti P, Boschero AC, Zoppi CC, Eizirik DL. FASEB J. 2018 Mar;32(3):1524-1536
IFN-α induces a preferential long-lasting expression of MHC class I in human pancreatic beta cells. Coomans de Brachène A, Dos Santos RS, Marroqui L, Colli ML, Marselli L, Mirmira RG, Marchetti P, Eizirik DL. Diabetologia. 2018 Mar;61(3):636-640
2017
PAX6 maintains β cell identity by repressing genes of alternative islet cell types. Swisa A, Avrahami D, Eden N, Zhang J, Feleke E, Dahan T, Cohen-Tayar Y, Stolovich-Rain M, Kaestner KH, Glaser B, Ashery-Padan R, Dor Y. J Clin Invest. 2017 Jan 3;127(1):230-243. doi: 10.1172/JCI88015. PMID: 27941241
Postprandial macrophage-derived IL-1β stimulates insulin, and both synergistically promote glucose disposal and inflammation. Dror E, Dalmas E, Meier DT, Wueest S, Thévenet J, Thienel C, Timper K, Nordmann TM, Traub S, Schulze F, Item F, Vallois D, Pattou F, Kerr-Conte J, Lavallard V, Berney T, Thorens B, Konrad D, Böni-Schnetzler M, Donath MY. Nat Immunol. 2017 Mar;18(3):283-292
Pancreatic β-cell protection from inflammatory stress by the endoplasmic reticulum proteins thrombospondin 1 and mesencephalic astrocyte-derived neutrotrophic factor (MANF). Cunha DA, Cito M, Grieco FA, Cosentino C, Danilova T, Ladrière L, Lindahl M, Domanskyi A, Bugliani M, Marchetti P, Eizirik DL, Cnop M. J Biol Chem. 2017 Sep 8;292(36):14977-14988
MicroRNAs miR-23a-3p, miR-23b-3p, and miR-149-5p Regulate the Expression of Proapoptotic BH3-Only Proteins DP5 and PUMA in Human Pancreatic β-Cells. Grieco FA, Sebastiani G, Juan-Mateu J, Villate O, Marroqui L, Ladrière L, Tugay K, Regazzi R, Bugliani M, Marchetti P, Dotta F, Eizirik DL. Diabetes. 2017 Jan;66(1):100-112.
Cytotoxic and regulatory roles of mucosal-associated invariant T cells in type 1 diabetes. Rouxel O, Da Silva J, Beaudoin L, Nel I, Tard C, Cagninacci L, Kiaf B, Oshima M, Diedisheim M, Salou M, Corbett A, Rossjohn J, McCluskey J, Scharfmann R, Battaglia M, Polak M, Lantz O, Beltrand J, Lehuen A. Nat Immunol. 2017 Dec;18(12):1321-1331.
Marroqui L, Dos Santos RS, Op de Beeck A, Coomans de Brachène A, Marselli L, Marchetti P, Eizirik DL. Diabetologia. 2017 Apr;60(4):656-667.
Interferon alpha impairs insulin production in human beta cells via endoplasmic reticulum stress. Lombardi A, Tomer Y. J Autoimmun. 2017 Jun;80:48-55.
MiR-335 overexpression impairs insulin secretion through defective priming of insulin vesicles. Salunkhe VA, Ofori JK, Gandasi NR, Salö SA, Hansson S, Andersson ME, Wendt A, Barg S, Esguerra JLS, Eliasson L. Physiol Rep. 2017 Nov;5(21). pii: e13493. doi: 10.14814/phy2.13493. PMID: 29122960.
Human Pancreatic β Cell lncRNAs Control Cell-Specific Regulatory Networks. Akerman I, Tu Z, Beucher A, Rolando DMY, Sauty-Colace C, Benazra M, Nakic N, Yang J, Wang H, Pasquali L, Moran I, Garcia-Hurtado J, Castro N, Gonzalez-Franco R, Stewart AF, Bonner C, Piemonti L, Berney T, Groop L, Kerr-Conte J, Pattou F, Argmann C, Schadt E, Ravassard P, Ferrer J. Cell Metab. 2017 Feb 7;25(2):400-411
Pannexin-2-deficiency sensitizes pancreatic β-cells to cytokine-induced apoptosis in vitro and impairs glucose tolerance in vivo. Berchtold LA, Miani M, Diep TA, Madsen AN, Cigliola V, Colli M, Krivokapic JM, Pociot F, Eizirik DL, Meda P, Holst B, Billestrup N, Størling J. Mol Cell Endocrinol. 2017 Jun 15;448:108-121.
Decreased STARD10 Expression Is Associated with Defective Insulin Secretion in Humans and Mice.
Carrat GR, Hu M, Nguyen-Tu MS, Chabosseau P, Gaulton KJ, van de Bunt M, Siddiq A, Falchi M, Thurner M, Canouil M, Pattou F, Leclerc I, Pullen TJ, Cane MC, Prabhala P, Greenwald W, Schulte A, Marchetti P, Ibberson M, MacDonald PE, Manning Fox JE, Gloyn AL, Froguel P, Solimena M, McCarthy MI, Rutter GA. Am J Hum Genet. 2017 Feb 2; 100(2): 238–256.
Neuron-enriched RNA-binding Proteins Regulate Pancreatic Beta Cell Function and Survival. Juan-Mateu J, Rech TH, Villate O, Lizarraga-Mollinedo E, Wendt A, Turatsinze JV, Brondani LA, Nardelli TR, Nogueira TC, Esguerra JL, Alvelos MI, Marchetti P, Eliasson L, Eizirik DL. J Biol Chem. 2017 Feb 24;292(8):3466-3480.
Dynamics of Insulin Secretion from EndoC-βH1 β-Cell Pseudoislets in Response to Glucose and Other Nutrient and Nonnutrient Secretagogues. Teraoku H, Lenzen S. J Diabetes Res. 2017;2017:2309630
Protective Role of Complement C3 Against Cytokine-Mediated β-Cell Apoptosis. Dos Santos RS, Marroqui , Grieco FA1, Marselli L, Suleiman M, Henz SR, Marchetti P, Wernersson R, Eizirik DL. Endocrinology. 2017 Aug 1;158(8):2503-2521
Addition of exogenous sodium palmitate increases the IAPP/insulin mRNA ratio via GPR40 in human EndoC-βH1 cells. Krizhanovskii C, Fred RG, Oskarsson ME, Westermark GT, Welsh N. Ups J Med Sci. 2017 Aug;122(3):149-159.
Sox5 regulates beta-cell phenotype and is reduced in type 2 diabetes. Axelsson AS, Mahdi T, Nenonen HA, Singh T, Hänzelmann S, Wendt A, Bagge A, Reinbothe TM, Millstein J, Yang X, Zhang B, Gusmao EG, Shu L, Szabat M, Tang Y, Wang J, Salö S, Eliasson L, Artner I, Fex M, Johnson JD, Wollheim CB, Derry JMJ, Mecham B, Spégel P, Mulder H, Costa IG, Zhang E, Rosengren AH. Nat Commun. 2017 Jun 6;8:15652.
Confluence does not affect the expression of miR-375 and its direct targets in rat and human insulin-secreting cell lines. Ofori JK, Malm HA, Mollet IG, Eliasson L, Esguerra JLS. Peer J. 2017 Jun 28;5:e3503.
A nanobody-based tracer targeting DPP6 for non-invasive imaging of human pancreatic endocrine cells. Balhuizen A, Massa S, Mathijs I, Turatsinze JV, De Vos J, Demine S, Xavier C, Villate O, Millard I, Egrise D, Capito C, Scharfmann R, In't Veld P, Marchetti P, Muyldermans S, Goldman S, Lahoutte T, Bouwens L, Eizirik DL, Devoogdt N. Sci Rep. 2017 Nov 9;7(1):15130.
Molecular phenotyping of multiple mouse strains under metabolic challenge uncovers a role for Elovl2 in glucose-induced insulin secretion. Cruciani-Guglielmacci C, Bellini L, Denom J, Oshima M, Fernandez N, Normandie-Levi P, Berney XP, Kassis N, Rouch C, Dairou J, Gorman T, Smith DM, Marley A, Liechti R, Kuznetsov D, Wigger L, Burdet F, Lefèvre AL, Wehrle I, Uphues I, Hildebrandt T, Rust W, Bernard C, Ktorza A, Rutter GA, Scharfmann R, Xenarios I, Le Stunff H, Thorens B, Magnan C, Ibberson M. Mol Metab. 2017 Jan 26;6(4):340-351
Orphan GPR61, GPR62 and GPR135 receptors and the melatonin MT2 receptor reciprocally modulate their signaling functions. Oishi A, Karamitri A, Gerbier R, Lahuna O, Ahmad R, Jockers R. Sci Rep. 2017 Aug 21;7(1):8990
An activating mutation in STAT3 results in neonatal diabetes through reduced insulin synthesis. Velayos T, Martínez R, Alonso M, Garcia-Etxebarria K, Aguayo A, Camarero C, Urrutia I, Martínez de LaPiscina I, Barrio R, Santin I, Castaño L. Diabetes. 2017 Apr;66(4):1022-1029.
The species origin of the cellular microenvironment influences markers of beta cell fate and function in EndoC-βH1 cells. Jeffery N, Richardson S, Beall C, Harries LW. Exp Cell Res. 2017 Dec 15;361(2):284-291
miR-204 is associated with an endocrine phenotype in human pancreatic islets but does not regulate the insulin mRNA through MAFA. Marzinotto I, Pellegrini S, Brigatti C, Nano R, Melzi R, Mercalli A, Liberati D, Sordi V, Ferrari M, Falconi M, Doglioni C, Ravassard P, Piemonti L, Lampasona V. Sci Rep. 2017 Oct 25;7(1):14051.
Chemistry and biology of reactive species with special reference to the antioxidative defence status in pancreatic β-cells. Lenzen S. Biochim Biophys Acta. 2017 Aug;1861(8):1929-1942
Basal hypersecretion of glucagon and insulin from palmitate-exposed human islets depends on FFAR1 but not decreased somatostatin secretion. Kristinsson H, Sargsyan E, Manell H, Smith DM, Göpel SO, Bergsten P. Sci Rep. 2017 Jul 5;7(1):4657.
Coxsackievirus-induced proteomic alterations in primary human islets provide insights for the etiology of diabetes. Nyalwidhe JO, Gallagher GR, Glenn LM, Morris MA, Vangala P, Jurczyk A, Bortell R, Harlan DM, Wang JP, Nadler JL. J Endocr Soc. 2017 Sep 11;1(10):1272-1286.
Expression and functional assessment of candidate type 2 diabetes susceptibility genes identify four new genes contributing to human insulin secretion. Ndiaye FK, Ortalli A, Canouil M, Huyvaert M, Salazar-Cardozo C, Lecoeur C, Verbanck M, Pawlowski V, Boutry R, Durand E, Rabearivelo I, Sand O, Marselli L, Kerr-Conte J, Chandra V, Scharfmann R, Poulain-Godefroy O, Marchetti P, Pattou F, Abderrahmani A, Froguel P, Bonnefond A. Mol Metab. 2017 Apr 8;6(6):459-470.
EndoC-βH1 cells display increased sensitivity to sodium palmitate when cultured in DMEM/F12 medium. Krizhanovskii C, Kristinsson H, Elksnis A, Wang X, Gavali H, Bergsten P, Scharfmann R, Welsh N. Islets. 2017 May 4;9(3):e1296995.
The monounsaturated fatty acid oleate is the major physiological toxic free fatty acid for human beta cells. Plötz T, Krümmel B, Laporte A, Pingitore A, Persaud SJ, Jörns A, Elsner M, Mehmeti I, Lenzen S. Nutr Diabetes. 2017 Dec 21;7(12):305
β-arrestin-2 is an essential regulator of pancreatic β-cell function under physiological and pathophysiological conditions. Zhu L, Almaça J, Dadi PK, Hong H, Sakamoto W, Rossi M, Lee RJ, Vierra NC, Lu H, Cui Y, McMillin SM, Perry NA, Gurevich VV, Lee A, Kuo B, Leapman RD, Matschinsky FM, Doliba NM, Urs NM, Caron MG, Jacobson DA, Caicedo A, Wess J.Nat Commun. 2017 Feb 1;8:14295. doi: 10.1038/ncomms14295. PMID: 28145434
2016
Identification of proliferative and mature β-cells in the islets of Langerhans. Bader E, Migliorini A, Gegg M, Moruzzi N, Gerdes J, Roscioni SS, Bakhti M, Brandl E, Irmler M, Beckers J, Aichler M, Feuchtinger A, Leitzinger C, Zischka H, Wang-Sattler R, Jastroch M, Tschöp M, Machicao F, Staiger H, Häring HU, Chmelova H, Chouinard JA, Oskolkov N, Korsgren O, Speier S, Lickert H.Nature. 2016 Jul 21;535(7612):430-4. Epub 2016 Jul 11. PMID: 27398620
Age-Dependent Pancreatic Gene Regulation Reveals Mechanisms Governing Human β Cell Function. Arda HE, Li L, Tsai J, Torre EA, Rosli Y, Peiris H1, Spitale RC, Dai C, Gu X, Qu K, Wang P, Wang J, Grompe M, Scharfmann R, Snyder MS, Bottino , Powers AC,Chang HY9, Kim SK. Cell Metab. 2016 May 10;23(5):909-20.
PTB and TIAR binding to insulin mRNA 3'- and 5'UTRs; implications for insulin biosynthesis and messenger stability. Fred RG, Mehrabi S, Adams CM, Welsh N. Heliyon. 2016 Sep 20;2(9):e00159. eCollection 2016 Sep. PMID: 27699280
Systematic Functional Characterization of Candidate Causal Genes for Type 2 Diabetes Risk Variants. Thomsen SK, Ceroni A, van de Bunt M, Burrows C, Barrett A, Scharfmann R, Ebner D, McCarthy MI, Gloyn AL. Diabetes. 2016 Dec;65(12):3805-3811.
MafA-Controlled Nicotinic Receptor Expression Is Essential for Insulin Secretion and Is Impaired in Patients with Type 2 Diabetes. Ganic E, Singh T, Luan C, Fadista J, Johansson JK, Cyphert HA, Bennet H, Storm P, Prost G, Ahlenius H, Renström E, Stein R, Groop L, Fex M, Artner I. Cell Rep. 2016 Mar 1;14(8):1991-2002
Sensitivity profile of the human EndoC-βH1 beta cell line to proinflammatory cytokines. Gurgul-Convey E, Mehmeti I, Plötz T, Jörns A, Lenzen S. Diabetologia. 2016 Oct;59(10):2125-33.
The non-canonical NF-κB pathway is induced by cytokines in pancreatic beta cells and contributes to cell death and proinflammatory responses in vitro. Meyerovich K, Fukaya M, Terra LF, Ortis F, Eizirik DL, Cardozo AK. Diabetologia. 2016 Mar;59(3):512-21.
Lack of Prox1 Downregulation Disrupts the Expansion and Maturation of Postnatal Murine β-Cells. Paul L, Walker EM, Drosos Y, Cyphert HA, Neale G, Stein R, South J, Grosveld G, Herrera PL, Sosa-Pineda B. Diabetes. 2016 Mar;65(3):687-98.
Innate and adaptive immunity to human beta cell lines: implications for beta cell therapy. van der Torren C, Zaldumbide A, Roelen DL, Duinkerken G, Brand-Schaaf SH, Peakman M, Czernichow P, Ravassard P, Scharfmann R, Roep BO. Diabetologia. 2016 Jan;59(1):170-5.
GPR44 is a pancreatic protein restricted to the human beta cell. Hellström-Lindahl E, Danielsson A, Ponten F, Czernichow P, Korsgren O, Johansson L, Eriksson O.Acta Diabetol. 2016 Jun;53(3):413-21.
Loss-of-Function Mutations in the Cell-Cycle Control Gene CDKN2A Impact on Glucose Homeostasis in Humans. Pal A, Potjer TP, Thomsen SK, Ng HJ, Barrett A, Scharfmann R, James TJ, Bishop DT, Karpe F, Godsland IF, Vasen HF, Newton-Bishop J, Pijl H, McCarthy MI, Gloyn AL. Diabetes. 2016 Feb;65(2):527-33.
Aggregation of Engineered Human β-Cells Into Pseudoislets: Insulin Secretion and Gene Expression Profile in Normoxic and Hypoxic Milieu. Lecomte MJ, Pechberty S, Machado C, Da Barroca S, Ravassard P, Scharfmann R, Czernichow P, Duvillié B.Cell Med. 2016 Aug 12;8(3):99-112
Xenotropic retrovirus Bxv1 in human pancreatic β cell lines. Kirkegaard JS, Ravassard P, Ingvarsen S, Diedisheim M, Bricout-Neveu E, Grønborg M, Frogne T, Scharfmann R, Madsen OD, Rescan C, Albagli O. J Clin Invest. 2016 Mar 1;126(3):1109-13.
p16(Ink4a)-induced senescence of pancreatic beta cells enhances insulin secretion. Helman A, Klochendler A, Azazmeh N, Gabai Y, Horwitz E, Anzi S, Swisa A, Condiotti R, Granit RZ, Nevo Y, Fixler Y, Shreibman D, Zamir A, Tornovsky-Babeay S, Dai C, Glaser B, Powers AC, Shapiro AM, Magnuson MA, Dor Y, Ben-Porath I. Nat Med. 2016 Apr;22(4):412-20
Extracellular acidification stimulates GPR68 mediated IL-8 production in human pancreatic β cells. Chandra V, Karamitri A, Richards P, Cormier F, Ramond C, Jockers R, Armanet M, Albagli-Curiel O, Scharfmann R. Sci Rep. 2016 May 11;6:25765.
Increased Expression of the Diabetes Gene SOX4 Reduces Insulin Secretion by Impaired Fusion Pore Expansion. Collins SC, Do HW, Hastoy B, Hugill A, Adam J, Chibalina MV, Galvanovskis J, Godazgar M, Lee S, Goldsworthy M, Salehi A, Tarasov AI, Rosengren AH, Cox R, Rorsman P. Diabetes. 2016 Jul;65(7):1952-61.
2015
Analysis artefacts of the INS-IGF2 fusion transcript. Wernersson R, Frogne T, Rescan C, Hansson L, Bruun C, Grønborg M, Jensen JN, Madsen OD. BMC Mol Biol. 2015 Jul 29;16:13.
TYK2, a Candidate Gene for Type 1 Diabetes, Modulates Apoptosis and the Innate Immune Response in Human Pancreatic β-Cells. Marroqui L, Dos Santos RS, Fløyel T, Grieco FA, Santin I, Op de Beeck A, Marselli L, Marchetti P, Pociot F, Eizirik DL. Diabetes. 2015 Nov;64(11):3808-17.
Accelerated Maturation of Human Stem Cell-Derived Pancreatic Progenitor Cells into Insulin-Secreting Cells in Immunodeficient Rats Relative to Mice. Bruin JE, Asadi A, Fox JK, Erener S, Rezania A, Kieffer TJ. Stem Cell Reports. 2015 Dec 8;5(6):1081-1096.
Role of the AMP kinase in cytokine-induced human EndoC-βH1 cell death. Fred RG, Kappe C, Ameur A, Cen J, Bergsten P, Ravassard P, Scharfmann R, Welsh N. Mol Cell Endocrinol. 2015 Oct 15;414:53-63.
Calcium signaling in a genetically engineered human pancreatic β-cell line.Krishnan K, Ma Z, Björklund A, Islam MS. Pancreas. 2015 Jul;44(5):773-7.
Dynamic recruitment of functionally distinct Swi/Snf chromatin remodeling complexes modulates Pdx1 activity in islet βcells. McKenna B, Guo M, Reynolds A, Hara M, Stein R. Cell Rep. 2015 Mar 31;10(12):2032-42.
DNA methylation directs functional maturation of pancreatic βcells.Dhawan S, Tschen SI, Zeng C, Guo T, Hebrok M, Matveyenko A, Bhushan A. J Clin Invest. 2015 Jul 1;125(7):2851-60.
MLL3 and MLL4 Methyltransferases Bind to the MAFA and MAFB Transcription Factors to Regulate Islet β-Cell Function. Scoville DW, Cyphert HA, Liao L, Xu J, Reynolds A, Guo S, Stein R. Diabetes. 2015 Nov;64(11):3772-83.
Loss of FFA2 and FFA3 increases insulin secretion and improves glucose tolerance in type 2 diabetes. Tang C, Ahmed K, Gille A, Lu S, Gröne HJ, Tunaru S, Offermanns S. Nat Med. 2015 Feb;21(2):173-7.
Cytokines induce endoplasmic reticulum stress in human, rat and mouse beta cells via different mechanisms. Brozzi F, Nardelli TR, Lopes M, Millard I, Barthson J, Igoillo-Esteve M, Grieco FA, Villate O, Oliveira JM, Casimir M, Bugliani M, Engin F, Hotamisligil GS, Marchetti P, Eizirik DL. Diabetologia. 2015 Oct;58(10):2307-16.
Distinct differences in the responses of the human pancreatic β-cell line EndoC-βH1 and human islets to proinflammatory cytokines. Oleson BJ, McGraw JA, Broniowska KA, Annamalai M, Chen J, Bushkofsky JR, Davis DB, Corbett JA, Mathews CE. Am J Physiol Regul Integr Comp Physiol. 2015 Sep;309(5):R525-34.
Physiological characterization of the human EndoC-βH1 β-cell line. Gurgul-Convey E, Kaminski MT, Lenzen S. Biochem Biophys Res Commun. 2015 Aug 14;464(1):13-9.
Genetic fine mapping and genomic annotation defines causal mechanisms at type 2 diabetes susceptibility loci. Gaulton KJ et al. Nat Genet. 2015 Dec;47(12):1415-25.
Brusatol inhibits the response of cultured beta-cells to pro-inflammatory cytokines in vitro. Turpaev K, Welsh N. Biochem Biophys Res Commun. 2015 May 8;460(3):868-72.
Imatinib mesylate stimulates low-density lipoprotein receptor-related protein 1-mediated ERK phosphorylation in insulin-producing cells. Fred RG, Boddeti SK, Lundberg M, Welsh N. Clin Sci (Lond). 2015 Jan;128(1):17-28.
Characterization of stimulus-secretion coupling in the human pancreatic EndoC-βH1 beta cell line. Andersson LE, Valtat B, Bagge A, Sharoyko VV, Nicholls DG, Ravassard P, Scharfmann R, Spégel P, Mulder H. PLoS One. 2015 Mar 24;10(3):e0120879.
A human beta cell line with drug inducible excision of immortalizing transgenes. Benazra M, Lecomte MJ, Colace C, Müller A, Machado C, Pechberty S, Bricout-Neveu E, Grenier-Godard M, Solimena M, Scharfmann R, Czernichow P, Ravassard P. Mol Metab. 2015 Oct 20;4(12):916-25
Accelerated Maturation of Human Stem Cell-Derived Pancreatic Progenitor Cells into Insulin-Secreting Cells in Immunodeficient Rats Relative to Mice. Bruin JE, Asadi A, Fox JK, Erener S, Rezania A, Kieffer TJ. Stem Cell Reports. 2015 Dec 8;5(6):1081-1096.
2014
Pancreatic islet enhancer clusters enriched in type 2 diabetes risk-associated variants. Pasquali L, Gaulton KJ, Rodríguez-Seguí SA, Mularoni L, Miguel-Escalada I, Akerman İ, Tena JJ, Morán I, Gómez-Marín C, van de Bunt M, Ponsa-Cobas J, Castro N, Nammo T, Cebola I, García-Hurtado J, Maestro MA, Pattou F, Piemonti L, Berney T, Gloyn AL, Ravassard P, Skarmeta JLG, Müller F, McCarthy MI, Ferrer J. Nat Genet. 2014 Feb;46(2):136-143.
IL-17A increases the expression of proinflammatory chemokines in human pancreatic islets. Grieco FA, Moore F, Vigneron F, Santin I, Villate O, Marselli L, Rondas D, Korf H, Overbergh L, Dotta F, Marchetti P, Mathieu C, Eizirik DL.Diabetologia. 2014 Mar;57(3):502-11
A combined "omics" approach identifies N-Myc interactor as a novel cytokine-induced regulator of IRE1 protein and c-Jun N-terminal kinase in pancreatic beta cells. Brozzi F, Gerlo S, Grieco FA, Nardelli TR, Lievens S, Gysemans C, Marselli L, Marchetti P, Mathieu C, Tavernier J, Eizirik DL. J Biol Chem. 2014 Jul 25;289(30):20677-93.
RFX6 regulates insulin secretion by modulating Ca2+ homeostasis in human β cells.Chandra V, Albagli-Curiel O, Hastoy B, Piccand J, Randriamampita C, Vaillant E, Cavé H, Busiah K, Froguel P, Vaxillaire M, Rorsman P, Polak M, Scharfmann R. Cell Rep. 2014 Dec 24;9(6):2206-18.
Human fucci pancreatic Beta cell lines: new tools to study Beta cell cycle and terminal differentiation. Carlier G, Maugein A, Cordier C, Pechberty S, Garfa-Traoré M, Martin P, Scharfmann R, Albagli O. PLoS One. 2014 Sep 26;9(9):e108202.
2013
Imatinib mesilate-induced phosphatidylinositol 3-kinase signalling and improved survival in insulin-producing cells: role of Src homology 2-containing inositol 5'-phosphatase interaction with c-Abl. Mokhtari D, Al-Amin A, Turpaev K, Li T, Idevall-Hagren O, Li J, Wuttke A, Fred RG, Ravassard P, Scharfmann R, Tengholm A, Welsh N. Diabetologia. 2013 Jun;56(6):1327-38.
2012
Human β cell transcriptome analysis uncovers lncRNAs that are tissue-specific, dynamically regulated, and abnormally expressed in type 2 diabetes. Morán I, Akerman I, van de Bunt M, Xie R, Benazra M, Nammo T, Arnes L, Nakić N, García-Hurtado J, Rodríguez-Seguí S, Pasquali L, Sauty-Colace C, Beucher A, Scharfmann R, van Arensbergen J, Johnson PR, Berry A, Lee C, Harkins T, Gmyr V, Pattou F, Kerr-Conte J, Piemonti L, Berney T, Hanley N, Gloyn AL, Sussel L, Langman L, Brayman KL, Sander M, McCarthy MI, Ravassard P, Ferrer J. Cell Metab. 2012 Oct 3;16(4):435-48.
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